@article{jansson2004structural, title={Structural basis for the dehalogenase activity in the haloalkane dehalogenase enzyme DhaA from Xanthobacter autotrophicus}, author={Jansson, Annelie and Ramaswamy, S and Mowbray, SL}, journal={Journal of Molecular Biology}, volume={337}, number={4}, pages={917--929}, year={2004}, publisher={Elsevier} } @article{schindler2009chemical, title={Chemical basis for the enhanced dehalogenase activity in the mutant DhaA haloalkane dehalogenase enzyme}, author={Schindler, Bettina and Stourac, Jan and Pavlova, Milena and Damborsky, Jiri}, journal={Journal of Biological Chemistry}, volume={284}, number={14}, pages={9118--9126}, year={2009}, publisher={American Society for Biochemistry and Molecular Biology} } @article{prokop2010structure, title={Structure and catalytic mechanism of the haloalkane dehalogenase DhaA31 with a novel substitution in the catalytic triad}, author={Prokop, Zbynek and Sykorova, Jana and Chaloupkova, Radka and Damborsky, Jiri}, journal={Proteins: Structure, Function, and Bioinformatics}, volume={78}, number={7}, pages={1454--1462}, year={2010}, publisher={Wiley Online Library} } @article{kalum2006substrate, title={Substrate specificity and stability of DhaA mutant enzymes}, author={Kalum, Lisbeth and Jones, JP and Oakes, Charles J and Schwarzenbacher, Robert}, journal={Biochemistry}, volume={45}, number={22}, pages={6826--6833}, year={2006}, publisher={American Chemical Society} } @article{stsiapanava2010characterization, title={Characterization and biotechnological potential of DhaA80, a haloalkane dehalogenase mutant with improved stability and activity}, author={Stsiapanava, Alice and Kurumbang, NP and Stourac, Jan and Chaloupkova, Radka and Damborsky, Jiri}, journal={Applied Microbiology and Biotechnology}, volume={85}, number={3}, pages={849--860}, year={2010}, publisher={Springer} } @article{chowdhury2009kinetic, title={Kinetic analysis of enzymatic dehalogenation using spectrophotometry}, author={Chowdhury, SK and others}, journal={Analytical Biochemistry}, volume={380}, pages={100--110}, year={2009}, publisher={Elsevier} } @article{damborsky2006haloalkane, title={Haloalkane dehalogenase: application in environmental biotechnology}, author={Damborsky, Jiri and Prokop, Zbynek}, journal={Biotechnology Advances}, volume={24}, pages={116--126}, year={2006}, publisher={Elsevier} } @article{stsiapanava2010characterization, title={Characterization and biotechnological potential of DhaA80, a haloalkane dehalogenase mutant with improved stability and activity}, author={Stsiapanava, Alice and others}, journal={Applied Microbiology and Biotechnology}, volume={85}, pages={849--860}, year={2010}, publisher={Springer} } @article{jansson2004structural, title={Structural basis for the dehalogenase activity in the haloalkane dehalogenase enzyme DhaA from Xanthobacter autotrophicus}, author={Jansson, Annelie and Ramaswamy, S and Mowbray, SL}, journal={Journal of Molecular Biology}, volume={337}, number={4}, pages={917--929}, year={2004}, publisher={Elsevier} } @article{schindler2009chemical, title={Chemical basis for the enhanced dehalogenase activity in the mutant DhaA haloalkane dehalogenase enzyme}, author={Schindler, Bettina and Stourac, Jan and Pavlova, Milena and Damborsky, Jiri}, journal={Journal of Biological Chemistry}, volume={284}, number={14}, pages={9118--9126}, year={2009}, publisher={American Society for Biochemistry and Molecular Biology} } @article{prokop2010structure, title={Structure and catalytic mechanism of the haloalkane dehalogenase DhaA31 with a novel substitution in the catalytic triad}, author={Prokop, Zbynek and Sykorova, Jana and Chaloupkova, Radka and Damborsky, Jiri}, journal={Proteins: Structure, Function, and Bioinformatics}, volume={78}, number={7}, pages={1454--1462}, year={2010}, publisher={Wiley Online Library} } @article{stsiapanava2010characterization, title={Characterization and biotechnological potential of DhaA80, a haloalkane dehalogenase mutant with improved stability and activity}, author={Stsiapanava, Alice and Kurumbang, NP and Stourac, Jan and Chaloupkova, Radka and Damborsky, Jiri}, journal={Applied Microbiology and Biotechnology}, volume={85}, number={3}, pages={849--860}, year={2010}, publisher={Springer} } @article{studier1990use, title={Use of T7 RNA polymerase to direct expression of cloned genes}, author={Studier, FW and Moffatt, BA}, journal={Journal of Molecular Biology}, volume={189}, pages={113--130}, year={1990}, publisher={Elsevier} } @book{sambrook1989molecular, title={Molecular Cloning: A Laboratory Manual}, author={Sambrook, J and Fritsch, EF and Maniatis, T}, edition={2nd}, year={1989}, publisher={Cold Spring Harbor Laboratory Press} } @manual{novagen1995, title={pET System Manual}, author={Novagen}, year={1995}, note={Madison, WI: Novagen, Inc.} } @article{petersen1994purification, title={Purification of recombinant proteins from Escherichia coli using histidine-tagged fusion proteins}, author={Petersen, G and Moller, LB}, journal={Journal of Chromatography A}, volume={658}, number={1}, pages={25--32}, year={1994}, publisher={Elsevier} } @article{de1997engineering, title={Engineering Escherichia coli BL21(DE3) strain for improved protein expression and stability}, author={De Lisa, MP and Bentley, WE}, journal={Biotechnology Progress}, volume={13}, number={4}, pages={547--555}, year={1997}, publisher={American Chemical Society} } @article{Studier1990, author = {Studier, F. W. and Moffatt, B. A.}, title = {Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes}, journal = {Journal of Molecular Biology}, volume = {189}, pages = {113--130}, year = {1986} } @article{Hochuli1988, author = {Hochuli, E. and Bannwarth, W. and Döbeli, H. and Gentz, R. and Stüber, D.}, title = {Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel Metal Chelate Adsorbent}, journal = {Nature Biotechnology}, volume = {6}, pages = {1321--1325}, year = {1988} } @article{Reynolds1986, author = {Reynolds, P. E. and Noble, W. C.}, title = {Properties of the Kanamycin Resistance Transposon Tn903}, journal = {Plasmid}, volume = {15}, pages = {19--28}, year = {1986} } @article{Sutcliffe1978, author = {Sutcliffe, J. G.}, title = {pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long}, journal = {Nucleic Acids Research}, volume = {5}, pages = {2721--2728}, year = {1978} } @article{Lewis2005, author = {Lewis, M.}, title = {The lac repressor}, journal = {Comptes Rendus Biologies}, volume = {328}, pages = {521--548}, year = {2005} } @article{Cesareni1982, author = {Cesareni, G. and Muesing, M. A. and Polisky, B.}, title = {Control of ColE1 DNA replication: the rop gene product negatively affects transcription from the replication primer promoter}, journal = {Proceedings of the National Academy of Sciences}, volume = {79}, pages = {6313--6317}, year = {1982} }