from Bio.Seq import Seq
from Bio.SeqRecord import SeqRecord
from Bio import SeqIO

# Sekwencje starterów
primer_ITS1 = "TCCGTAGGTGAACCTGCGG"
primer_ITS4 = "TCCTCCGCTTATTGATATGC"

# Tworzenie obiektów SeqRecord dla starterów
its1_record = SeqRecord(Seq(primer_ITS1), id="ITS1", description="ITS1 primer sequence")
its4_record = SeqRecord(Seq(primer_ITS4), id="ITS4", description="ITS4 primer sequence")

# Zapis do plików FASTA w katalogu ../data
with open("../data/ITS1.fasta", "w") as its1_file:
    SeqIO.write(its1_record, its1_file, "fasta")

with open("../data/ITS4.fasta", "w") as its4_file:
    SeqIO.write(its4_record, its4_file, "fasta")

print("Sekwencje starterów zapisano do plików ../data/ITS1.fasta i ../data/ITS4.fasta.")