57 lines
2.2 KiB
TeX
57 lines
2.2 KiB
TeX
\documentclass[a4paper,12pt]{article}
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\usepackage{amsmath}
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\usepackage{geometry}
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\usepackage{fancyhdr}
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\usepackage{graphicx}
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\usepackage{enumitem} % For custom enumeration
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% Page setup
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\geometry{left=2.5cm, right=2.5cm, top=2.5cm, bottom=2.5cm}
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\pagestyle{fancy}
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% Header and Footer
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\lhead{PCz, Biotechnology, Plant In Vitro Cultures 2024}
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\rhead{Group I}
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\lfoot{Commit UUID with timestamp}
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\rfoot{\thepage}
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% Document title
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\title{\textbf{Practical 2}\\ Establishment of axenic culture of plants as explant source for \textit{in vitro} experiments}
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\author{A. Gorbelak}
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\date{}
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% Define new list type longenum with five levels
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\newlist{longenum}{enumerate}{5}
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\setlist[longenum,1]{label=\arabic*., left=0.5em}
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\setlist[longenum,2]{label=\arabic*), left=1em}
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\setlist[longenum,3]{label=\alph*., left=1.5em}
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\setlist[longenum,4]{label=\alph*), left=2em}
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\setlist[longenum,5]{label=--, left=3.5em}
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\begin{document}
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\maketitle
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\section*{Objectives}
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\begin{longenum}
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\item To learn methods of plant material sterilisation.
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\item To gain experience in the establishment and maintenance of sterile cultures of seedlings and plants.
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\item To establish a sterile culture of \textit{Arabidopsis thaliana} and \textit{Nicotiana sp.} plants used as an explant source in Practicals 3, 7, and 8.
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\end{longenum}
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\section*{Introduction}
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\begin{longenum}
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\item To avoid the process of plant material sterilisation, sterile cultures of plants growing in \textit{in vitro} conditions are recommended as a source of explants.
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\item When seedling fragments are used as explants, seeds can be sterilised.
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\item Seeds can be germinated \textit{in vitro}, providing a sterile seedling culture.
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\item When explants are taken from mature plant tissue, donor plants are typically not maintained in sterile culture.
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\item In such cases, sterilisation is necessary.
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\item An exception includes species from the \textit{Solanaceae} family (e.g., tomato, tobacco).
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\item These plants are easily maintained in \textit{in vitro} culture through propagation by "cuttings."
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\item These cuttings are grown on MS10 agar medium.
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\item Plants from germinated \textit{in vitro} seeds of the \textit{Solanaceae} family can be propagated by cuttings.
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\end{longenum}
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\end{document}
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