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+ +\begin{document} + +\maketitle + +\section*{Objectives} +\begin{itemize} + \item To learn methods of plant material sterilisation. + \item To gain experience in establishment and maintenance of sterile culture of seedlings and plants. + \item To establish a sterile culture of \textit{Arabidopsis thaliana} and \textit{Nicotiana sp.} plants used as an explant source in Practicals 3, 7, and 8. +\end{itemize} + +\section*{Introduction} +To avoid the process of plant material sterilisation, sterile culture of plants growing in \textit{in vitro} conditions are recommended as a source of the explants. When fragments of seedlings are used as the explants, seeds can be sterilised and germinated \textit{in vitro}, providing axenic seedling culture. + +In the case the explants are taken from mature plant tissue, usually the donor plants cannot be maintained in axenic culture, and the process of sterilisation cannot be avoided. + +One of the exceptions are the species from the \textit{Solanaceae} family (e.g., tomato, tobacco), which are easily maintained in \textit{in vitro} culture by a propagation of so-called "cuttings" cultured on an MS10 agar medium. Plants from germinated \textit{in vitro} seeds of \textit{Solanaceae} family can be propagated by cuttings. +\end{document} +