commit b96809e2ba76fc2250f4a75dde3047a37120a844
Author: baiobelfer <baiobelfer@gmail.com>
Date:   Sat Oct 26 19:03:51 2024 +0200

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+\documentclass[a4paper,12pt]{article}
+
+\usepackage{amsmath}
+\usepackage{geometry}
+\usepackage{fancyhdr}
+\usepackage{graphicx}
+
+% Page setup
+\geometry{left=2.5cm, right=2.5cm, top=2.5cm, bottom=2.5cm}
+\pagestyle{fancy}
+
+% Header and Footer
+\lhead{Biotechnologia PCz 2024}
+\rhead{Grupa I}
+\lfoot{Commit UUID with timestamp}
+\rfoot{\thepage}
+
+% Document title
+\title{\textbf{Practical 2}\\ Establishment of axenic culture of plants as explant source for \textit{in vitro} experiments}
+\author{M.D. Gaj}
+\date{}
+
+\begin{document}
+
+\maketitle
+
+\section*{Objectives}
+\begin{itemize}
+    \item To learn methods of plant material sterilisation.
+    \item To gain experience in establishment and maintenance of sterile culture of seedlings and plants.
+    \item To establish a sterile culture of \textit{Arabidopsis thaliana} and \textit{Nicotiana sp.} plants used as an explant source in Practicals 3, 7, and 8.
+\end{itemize}
+
+\section*{Introduction}
+To avoid the process of plant material sterilisation, sterile culture of plants growing in \textit{in vitro} conditions are recommended as a source of the explants. When fragments of seedlings are used as the explants, seeds can be sterilised and germinated \textit{in vitro}, providing axenic seedling culture.
+
+In the case the explants are taken from mature plant tissue, usually the donor plants cannot be maintained in axenic culture, and the process of sterilisation cannot be avoided.
+
+One of the exceptions are the species from the \textit{Solanaceae} family (e.g., tomato, tobacco), which are easily maintained in \textit{in vitro} culture by a propagation of so-called "cuttings" cultured on an MS10 agar medium. Plants from germinated \textit{in vitro} seeds of \textit{Solanaceae} family can be propagated by cuttings.
+\end{document}
+