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baiobelfer 2024-10-26 19:22:46 +02:00
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\usepackage{fancyhdr}
\usepackage{graphicx}
\usepackage{enumitem} % For custom enumeration
\usepackage{cite} % For citations
\usepackage{hyperref} % For hyperlinks
\usepackage[
sortcites,
backend=biber,
hyperref=true,
firstinits=true,
maxbibnames=99,
]{biblatex}
\addbibresource{references.bib}
% Page setup
\geometry{left=2.5cm, right=2.5cm, top=2.5cm, bottom=2.5cm}
\pagestyle{fancy}
% Header and Footer
\lhead{PCz, Biotechnology, Plant In Vitro Cultures 2024}
\lhead{PCz, Biotechnology, Plant \textit{In Vitro} Cultures 2024}
\rhead{Group I}
\lfoot{Commit UUID with timestamp}
\rfoot{\thepage}
% Document title
\title{\textbf{Practical 2}\\ Establishment of axenic culture of plants as explant source for \textit{in vitro} experiments}
\title{\textbf{Practical 2}\\ Establishment of Axenic Plant Cultures as Explant Sources for \textit{In Vitro} Experiments}
\author{A. Gorbelak}
\date{}
@ -35,22 +46,26 @@
\section*{Objectives}
\begin{longenum}
\item To learn methods of plant material sterilisation.
\item To learn methods of plant material sterilization.
\item To gain experience in the establishment and maintenance of sterile cultures of seedlings and plants.
\item To establish a sterile culture of \textit{Arabidopsis thaliana} and \textit{Nicotiana sp.} plants used as an explant source in Practicals 3, 7, and 8.
\item To establish sterile cultures of \textit{Arabidopsis thaliana} and \textit{Nicotiana sp.} as explant sources for future practicals.
\end{longenum}
\section*{Introduction}
\begin{longenum}
\item To avoid the process of plant material sterilisation, sterile cultures of plants growing in \textit{in vitro} conditions are recommended as a source of explants.
\item When seedling fragments are used as explants, seeds can be sterilised.
\item Seeds can be germinated \textit{in vitro}, providing a sterile seedling culture.
\item When explants are taken from mature plant tissue, donor plants are typically not maintained in sterile culture.
\item In such cases, sterilisation is necessary.
\item An exception includes species from the \textit{Solanaceae} family (e.g., tomato, tobacco).
\item These plants are easily maintained in \textit{in vitro} culture through propagation by "cuttings."
\item These cuttings are grown on MS10 agar medium.
\item Plants from germinated \textit{in vitro} seeds of the \textit{Solanaceae} family can be propagated by cuttings.
\item Sterile \textit{in vitro} plant cultures are recommended as sources of explants to avoid repeated sterilization of plant material \cite{George2008}.
\item When seedling fragments are used as explants, seeds can be sterilized and germinated \textit{in vitro}, providing axenic seedling cultures.
\item For explants taken from mature plant tissues, donor plants are typically not maintained in sterile culture, necessitating surface sterilization of the explant material \cite{Cassells2012}.
\item An exception includes species from the \textit{Solanaceae} family (e.g., tomato, tobacco), which are easily maintained in \textit{in vitro} cultures through propagation by cuttings.
\item These cuttings are grown on Murashige and Skoog (MS) medium \cite{Murashige1962}, commonly supplemented with 1\% sucrose (MS10).
\item Plants from germinated \textit{in vitro} seeds of the \textit{Solanaceae} family can be propagated by cuttings, providing a continuous source of sterile explant material.
\end{longenum}
\bibliographystyle{plain}
\bibliography{references}
\newpage
\printbibliography
\end{document}