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b/main.tex @@ -5,19 +5,30 @@ \usepackage{fancyhdr} \usepackage{graphicx} \usepackage{enumitem} % For custom enumeration +\usepackage{cite} % For citations +\usepackage{hyperref} % For hyperlinks + +\usepackage[ + sortcites, + backend=biber, + hyperref=true, + firstinits=true, + maxbibnames=99, + ]{biblatex} +\addbibresource{references.bib} % Page setup \geometry{left=2.5cm, right=2.5cm, top=2.5cm, bottom=2.5cm} \pagestyle{fancy} % Header and Footer -\lhead{PCz, Biotechnology, Plant In Vitro Cultures 2024} +\lhead{PCz, Biotechnology, Plant \textit{In Vitro} Cultures 2024} \rhead{Group I} \lfoot{Commit UUID with timestamp} \rfoot{\thepage} % Document title -\title{\textbf{Practical 2}\\ Establishment of axenic culture of plants as explant source for \textit{in vitro} experiments} +\title{\textbf{Practical 2}\\ Establishment of Axenic Plant Cultures as Explant Sources for \textit{In Vitro} Experiments} \author{A. Gorbelak} \date{} @@ -35,22 +46,26 @@ \section*{Objectives} \begin{longenum} - \item To learn methods of plant material sterilisation. + \item To learn methods of plant material sterilization. \item To gain experience in the establishment and maintenance of sterile cultures of seedlings and plants. - \item To establish a sterile culture of \textit{Arabidopsis thaliana} and \textit{Nicotiana sp.} plants used as an explant source in Practicals 3, 7, and 8. + \item To establish sterile cultures of \textit{Arabidopsis thaliana} and \textit{Nicotiana sp.} as explant sources for future practicals. \end{longenum} \section*{Introduction} \begin{longenum} - \item To avoid the process of plant material sterilisation, sterile cultures of plants growing in \textit{in vitro} conditions are recommended as a source of explants. - \item When seedling fragments are used as explants, seeds can be sterilised. - \item Seeds can be germinated \textit{in vitro}, providing a sterile seedling culture. - \item When explants are taken from mature plant tissue, donor plants are typically not maintained in sterile culture. - \item In such cases, sterilisation is necessary. - \item An exception includes species from the \textit{Solanaceae} family (e.g., tomato, tobacco). - \item These plants are easily maintained in \textit{in vitro} culture through propagation by "cuttings." - \item These cuttings are grown on MS10 agar medium. - \item Plants from germinated \textit{in vitro} seeds of the \textit{Solanaceae} family can be propagated by cuttings. + \item Sterile \textit{in vitro} plant cultures are recommended as sources of explants to avoid repeated sterilization of plant material \cite{George2008}. + \item When seedling fragments are used as explants, seeds can be sterilized and germinated \textit{in vitro}, providing axenic seedling cultures. + \item For explants taken from mature plant tissues, donor plants are typically not maintained in sterile culture, necessitating surface sterilization of the explant material \cite{Cassells2012}. + \item An exception includes species from the \textit{Solanaceae} family (e.g., tomato, tobacco), which are easily maintained in \textit{in vitro} cultures through propagation by cuttings. + \item These cuttings are grown on Murashige and Skoog (MS) medium \cite{Murashige1962}, commonly supplemented with 1\% sucrose (MS10). + \item Plants from germinated \textit{in vitro} seeds of the \textit{Solanaceae} family can be propagated by cuttings, providing a continuous source of sterile explant material. \end{longenum} +\bibliographystyle{plain} +\bibliography{references} + +\newpage +\printbibliography + \end{document} +