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zP=faV-}3hqjDJDFX?b|~|J7s>4Yp#1nNxl|7bV=5{eU+I{PCWEz@NOoI^Poq!}oha zLP7>9Ia5_$=p#wH*dSHNsH`0P#`m9_4C$&|*%kqGw&&Zl3g?X9Uu$1`94)c@+QIY^ z4vr)9e=@7gIyC@soI>8~zE!Q;FdQxnpNn~X_y7Ge$!p^{7{WX}Ky*e%d5yQ|{{@1L Bj&T40 diff --git a/main.tex b/main.tex index 5593f67..9fbdd2d 100644 --- a/main.tex +++ b/main.tex @@ -4,38 +4,53 @@ \usepackage{geometry} \usepackage{fancyhdr} \usepackage{graphicx} +\usepackage{enumitem} % For custom enumeration % Page setup \geometry{left=2.5cm, right=2.5cm, top=2.5cm, bottom=2.5cm} \pagestyle{fancy} % Header and Footer -\lhead{Biotechnologia PCz 2024} -\rhead{Grupa I} +\lhead{PCz, Biotechnology, Plant In Vitro Cultures 2024} +\rhead{Group I} \lfoot{Commit UUID with timestamp} \rfoot{\thepage} % Document title \title{\textbf{Practical 2}\\ Establishment of axenic culture of plants as explant source for \textit{in vitro} experiments} -\author{M.D. Gaj} +\author{A. Gorbelak} \date{} +% Define new list type longenum with five levels +\newlist{longenum}{enumerate}{5} +\setlist[longenum,1]{label=\arabic*., left=0.5em} +\setlist[longenum,2]{label=\arabic*), left=1em} +\setlist[longenum,3]{label=\alph*., left=1.5em} +\setlist[longenum,4]{label=\alph*), left=2em} +\setlist[longenum,5]{label=--, left=3.5em} + \begin{document} \maketitle \section*{Objectives} -\begin{itemize} +\begin{longenum} \item To learn methods of plant material sterilisation. - \item To gain experience in establishment and maintenance of sterile culture of seedlings and plants. + \item To gain experience in the establishment and maintenance of sterile cultures of seedlings and plants. \item To establish a sterile culture of \textit{Arabidopsis thaliana} and \textit{Nicotiana sp.} plants used as an explant source in Practicals 3, 7, and 8. -\end{itemize} +\end{longenum} \section*{Introduction} -To avoid the process of plant material sterilisation, sterile culture of plants growing in \textit{in vitro} conditions are recommended as a source of the explants. When fragments of seedlings are used as the explants, seeds can be sterilised and germinated \textit{in vitro}, providing axenic seedling culture. +\begin{longenum} + \item To avoid the process of plant material sterilisation, sterile cultures of plants growing in \textit{in vitro} conditions are recommended as a source of explants. + \item When seedling fragments are used as explants, seeds can be sterilised. + \item Seeds can be germinated \textit{in vitro}, providing a sterile seedling culture. + \item When explants are taken from mature plant tissue, donor plants are typically not maintained in sterile culture. + \item In such cases, sterilisation is necessary. + \item An exception includes species from the \textit{Solanaceae} family (e.g., tomato, tobacco). + \item These plants are easily maintained in \textit{in vitro} culture through propagation by "cuttings." + \item These cuttings are grown on MS10 agar medium. + \item Plants from germinated \textit{in vitro} seeds of the \textit{Solanaceae} family can be propagated by cuttings. +\end{longenum} -In the case the explants are taken from mature plant tissue, usually the donor plants cannot be maintained in axenic culture, and the process of sterilisation cannot be avoided. - -One of the exceptions are the species from the \textit{Solanaceae} family (e.g., tomato, tobacco), which are easily maintained in \textit{in vitro} culture by a propagation of so-called "cuttings" cultured on an MS10 agar medium. Plants from germinated \textit{in vitro} seeds of \textit{Solanaceae} family can be propagated by cuttings. \end{document} -