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@@ -4,38 +4,53 @@
 \usepackage{geometry}
 \usepackage{fancyhdr}
 \usepackage{graphicx}
+\usepackage{enumitem} % For custom enumeration
 
 % Page setup
 \geometry{left=2.5cm, right=2.5cm, top=2.5cm, bottom=2.5cm}
 \pagestyle{fancy}
 
 % Header and Footer
-\lhead{Biotechnologia PCz 2024}
-\rhead{Grupa I}
+\lhead{PCz, Biotechnology, Plant In Vitro Cultures 2024}
+\rhead{Group I}
 \lfoot{Commit UUID with timestamp}
 \rfoot{\thepage}
 
 % Document title
 \title{\textbf{Practical 2}\\ Establishment of axenic culture of plants as explant source for \textit{in vitro} experiments}
-\author{M.D. Gaj}
+\author{A. Gorbelak}
 \date{}
 
+% Define new list type longenum with five levels
+\newlist{longenum}{enumerate}{5}
+\setlist[longenum,1]{label=\arabic*., left=0.5em}
+\setlist[longenum,2]{label=\arabic*), left=1em}
+\setlist[longenum,3]{label=\alph*., left=1.5em}
+\setlist[longenum,4]{label=\alph*), left=2em}
+\setlist[longenum,5]{label=--, left=3.5em}
+
 \begin{document}
 
 \maketitle
 
 \section*{Objectives}
-\begin{itemize}
+\begin{longenum}
     \item To learn methods of plant material sterilisation.
-    \item To gain experience in establishment and maintenance of sterile culture of seedlings and plants.
+    \item To gain experience in the establishment and maintenance of sterile cultures of seedlings and plants.
     \item To establish a sterile culture of \textit{Arabidopsis thaliana} and \textit{Nicotiana sp.} plants used as an explant source in Practicals 3, 7, and 8.
-\end{itemize}
+\end{longenum}
 
 \section*{Introduction}
-To avoid the process of plant material sterilisation, sterile culture of plants growing in \textit{in vitro} conditions are recommended as a source of the explants. When fragments of seedlings are used as the explants, seeds can be sterilised and germinated \textit{in vitro}, providing axenic seedling culture.
+\begin{longenum}
+    \item To avoid the process of plant material sterilisation, sterile cultures of plants growing in \textit{in vitro} conditions are recommended as a source of explants.
+    \item When seedling fragments are used as explants, seeds can be sterilised.
+    \item Seeds can be germinated \textit{in vitro}, providing a sterile seedling culture.
+    \item When explants are taken from mature plant tissue, donor plants are typically not maintained in sterile culture.
+    \item In such cases, sterilisation is necessary.
+    \item An exception includes species from the \textit{Solanaceae} family (e.g., tomato, tobacco).
+    \item These plants are easily maintained in \textit{in vitro} culture through propagation by "cuttings."
+    \item These cuttings are grown on MS10 agar medium.
+    \item Plants from germinated \textit{in vitro} seeds of the \textit{Solanaceae} family can be propagated by cuttings.
+\end{longenum}
 
-In the case the explants are taken from mature plant tissue, usually the donor plants cannot be maintained in axenic culture, and the process of sterilisation cannot be avoided.
-
-One of the exceptions are the species from the \textit{Solanaceae} family (e.g., tomato, tobacco), which are easily maintained in \textit{in vitro} culture by a propagation of so-called "cuttings" cultured on an MS10 agar medium. Plants from germinated \textit{in vitro} seeds of \textit{Solanaceae} family can be propagated by cuttings.
 \end{document}
-