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baiobelfer 2024-10-26 19:15:29 +02:00
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\usepackage{geometry}
\usepackage{fancyhdr}
\usepackage{graphicx}
\usepackage{enumitem} % For custom enumeration
% Page setup
\geometry{left=2.5cm, right=2.5cm, top=2.5cm, bottom=2.5cm}
\pagestyle{fancy}
% Header and Footer
\lhead{Biotechnologia PCz 2024}
\rhead{Grupa I}
\lhead{PCz, Biotechnology, Plant In Vitro Cultures 2024}
\rhead{Group I}
\lfoot{Commit UUID with timestamp}
\rfoot{\thepage}
% Document title
\title{\textbf{Practical 2}\\ Establishment of axenic culture of plants as explant source for \textit{in vitro} experiments}
\author{M.D. Gaj}
\author{A. Gorbelak}
\date{}
% Define new list type longenum with five levels
\newlist{longenum}{enumerate}{5}
\setlist[longenum,1]{label=\arabic*., left=0.5em}
\setlist[longenum,2]{label=\arabic*), left=1em}
\setlist[longenum,3]{label=\alph*., left=1.5em}
\setlist[longenum,4]{label=\alph*), left=2em}
\setlist[longenum,5]{label=--, left=3.5em}
\begin{document}
\maketitle
\section*{Objectives}
\begin{itemize}
\begin{longenum}
\item To learn methods of plant material sterilisation.
\item To gain experience in establishment and maintenance of sterile culture of seedlings and plants.
\item To gain experience in the establishment and maintenance of sterile cultures of seedlings and plants.
\item To establish a sterile culture of \textit{Arabidopsis thaliana} and \textit{Nicotiana sp.} plants used as an explant source in Practicals 3, 7, and 8.
\end{itemize}
\end{longenum}
\section*{Introduction}
To avoid the process of plant material sterilisation, sterile culture of plants growing in \textit{in vitro} conditions are recommended as a source of the explants. When fragments of seedlings are used as the explants, seeds can be sterilised and germinated \textit{in vitro}, providing axenic seedling culture.
\begin{longenum}
\item To avoid the process of plant material sterilisation, sterile cultures of plants growing in \textit{in vitro} conditions are recommended as a source of explants.
\item When seedling fragments are used as explants, seeds can be sterilised.
\item Seeds can be germinated \textit{in vitro}, providing a sterile seedling culture.
\item When explants are taken from mature plant tissue, donor plants are typically not maintained in sterile culture.
\item In such cases, sterilisation is necessary.
\item An exception includes species from the \textit{Solanaceae} family (e.g., tomato, tobacco).
\item These plants are easily maintained in \textit{in vitro} culture through propagation by "cuttings."
\item These cuttings are grown on MS10 agar medium.
\item Plants from germinated \textit{in vitro} seeds of the \textit{Solanaceae} family can be propagated by cuttings.
\end{longenum}
In the case the explants are taken from mature plant tissue, usually the donor plants cannot be maintained in axenic culture, and the process of sterilisation cannot be avoided.
One of the exceptions are the species from the \textit{Solanaceae} family (e.g., tomato, tobacco), which are easily maintained in \textit{in vitro} culture by a propagation of so-called "cuttings" cultured on an MS10 agar medium. Plants from germinated \textit{in vitro} seeds of \textit{Solanaceae} family can be propagated by cuttings.
\end{document}