42 lines
1.7 KiB
TeX
42 lines
1.7 KiB
TeX
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\documentclass[a4paper,12pt]{article}
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\usepackage{amsmath}
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\usepackage{geometry}
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\usepackage{fancyhdr}
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\usepackage{graphicx}
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% Page setup
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\geometry{left=2.5cm, right=2.5cm, top=2.5cm, bottom=2.5cm}
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\pagestyle{fancy}
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% Header and Footer
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\lhead{Biotechnologia PCz 2024}
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\rhead{Grupa I}
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\lfoot{Commit UUID with timestamp}
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\rfoot{\thepage}
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% Document title
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\title{\textbf{Practical 2}\\ Establishment of axenic culture of plants as explant source for \textit{in vitro} experiments}
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\author{M.D. Gaj}
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\date{}
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\begin{document}
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\maketitle
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\section*{Objectives}
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\begin{itemize}
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\item To learn methods of plant material sterilisation.
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\item To gain experience in establishment and maintenance of sterile culture of seedlings and plants.
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\item To establish a sterile culture of \textit{Arabidopsis thaliana} and \textit{Nicotiana sp.} plants used as an explant source in Practicals 3, 7, and 8.
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\end{itemize}
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\section*{Introduction}
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To avoid the process of plant material sterilisation, sterile culture of plants growing in \textit{in vitro} conditions are recommended as a source of the explants. When fragments of seedlings are used as the explants, seeds can be sterilised and germinated \textit{in vitro}, providing axenic seedling culture.
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In the case the explants are taken from mature plant tissue, usually the donor plants cannot be maintained in axenic culture, and the process of sterilisation cannot be avoided.
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One of the exceptions are the species from the \textit{Solanaceae} family (e.g., tomato, tobacco), which are easily maintained in \textit{in vitro} culture by a propagation of so-called "cuttings" cultured on an MS10 agar medium. Plants from germinated \textit{in vitro} seeds of \textit{Solanaceae} family can be propagated by cuttings.
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\end{document}
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