cinv-instr/main.tex

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\documentclass[a4paper,12pt]{article}
\usepackage{amsmath}
\usepackage{geometry}
\usepackage{fancyhdr}
\usepackage{graphicx}
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\usepackage{enumitem} % For custom enumeration
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\usepackage{cite} % For citations
\usepackage{hyperref} % For hyperlinks
\usepackage[
sortcites,
backend=biber,
hyperref=true,
firstinits=true,
maxbibnames=99,
]{biblatex}
\addbibresource{references.bib}
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% Page setup
\geometry{left=2.5cm, right=2.5cm, top=2.5cm, bottom=2.5cm}
\pagestyle{fancy}
% Header and Footer
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\lhead{PCz, Biotechnology, Plant \textit{In Vitro} Cultures 2024}
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\rhead{Group I}
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\lfoot{Commit UUID with timestamp}
\rfoot{\thepage}
% Document title
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\title{\textbf{Practical 2}\\ Establishment of Axenic Plant Cultures as Explant Sources for \textit{In Vitro} Experiments}
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\author{A. Gorbelak}
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\date{}
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% Define new list type longenum with five levels
\newlist{longenum}{enumerate}{5}
\setlist[longenum,1]{label=\arabic*., left=0.5em}
\setlist[longenum,2]{label=\arabic*), left=1em}
\setlist[longenum,3]{label=\alph*., left=1.5em}
\setlist[longenum,4]{label=\alph*), left=2em}
\setlist[longenum,5]{label=--, left=3.5em}
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\begin{document}
\maketitle
\section*{Objectives}
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\begin{longenum}
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\item To learn methods of plant material sterilization.
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\item To gain experience in the establishment and maintenance of sterile cultures of seedlings and plants.
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\item To establish sterile cultures of \textit{Arabidopsis thaliana} and \textit{Nicotiana sp.} as explant sources for future practicals.
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\end{longenum}
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\section*{Introduction}
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\begin{longenum}
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\item Sterile \textit{in vitro} plant cultures are recommended as sources of explants to avoid repeated sterilization of plant material \cite{George2008}.
\item When seedling fragments are used as explants, seeds can be sterilized and germinated \textit{in vitro}, providing axenic seedling cultures.
\item For explants taken from mature plant tissues, donor plants are typically not maintained in sterile culture, necessitating surface sterilization of the explant material \cite{Cassells2012}.
\item An exception includes species from the \textit{Solanaceae} family (e.g., tomato, tobacco), which are easily maintained in \textit{in vitro} cultures through propagation by cuttings.
\item These cuttings are grown on Murashige and Skoog (MS) medium \cite{Murashige1962}, commonly supplemented with 1\% sucrose (MS10).
\item Plants from germinated \textit{in vitro} seeds of the \textit{Solanaceae} family can be propagated by cuttings, providing a continuous source of sterile explant material.
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\end{longenum}
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\bibliographystyle{plain}
\bibliography{references}
\newpage
\printbibliography
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\end{document}
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