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No commits in common. "mpabi" and "crispr" have entirely different histories.
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49
doc/main.tex
49
doc/main.tex
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@ -37,21 +37,12 @@
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% \setlist[longenum,2]{label=\arabic*), nosep}
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% \setlist[longenum,3]{label=\alph*., nosep}
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% Definiowanie nowego typu listy 'longenum' z pięcioma poziomami numeracji
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% Pakiet do nagłówków i stopek
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@ -74,6 +65,20 @@
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% Definicje kolorów
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@ -107,6 +112,8 @@
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% Ustawienie stylu jako domyślnego
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||||
\lstset{style=mystyle}
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% Definicja nowej komendy \zs
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\newcommand{\zs}{\textcolor{zs}{Zespół Szkół }}
|
||||
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||||
% Wczytanie pliku z informacjami o commit
|
||||
\input{commit.tex}
|
||||
|
|
@ -141,21 +148,9 @@
|
|||
\label{sec:crispr}
|
||||
|
||||
\newpage
|
||||
\input{modules/arabidopsis}
|
||||
\input{modules/bio}
|
||||
\label{sec:bio}
|
||||
|
||||
\newpage
|
||||
\input{modules/alg}
|
||||
\label{sec:bio}
|
||||
|
||||
\newpage
|
||||
\input{modules/protocol}
|
||||
\label{sec:protocol}
|
||||
|
||||
% \newpage
|
||||
% \input{modules/bio}
|
||||
% \label{sec:bio}
|
||||
|
||||
% \newpage
|
||||
% \input{modules/wymagania_edukacyjne} % Wczytaj zawartość pliku
|
||||
% \label{sec:wymagania_edukacyjne}
|
||||
|
|
|
|||
|
|
@ -1,52 +0,0 @@
|
|||
\item
|
||||
\begin{center}
|
||||
\fbox{
|
||||
\begin{minipage}{0.9\textwidth}
|
||||
\textbf{Modyfikacja genetyczna poprawiająca odporność na suszę}.
|
||||
\end{minipage}
|
||||
}
|
||||
\end{center}
|
||||
\section*
|
||||
|
||||
\begin{longenum}
|
||||
\item Wprowadzenie
|
||||
\begin{longenum}
|
||||
\item Stosowanie CRISPR w roślinach pozwala na precyzyjne wprowadzenie modyfikacji genetycznych, które mogą poprawić odporność na stresy abiotyczne, takie jak susza.
|
||||
\item Zwiększenie odporności Arabidopsis na suszę mogłoby zostać osiągnięte przez ukierunkowaną modyfikację genów, które regulują odpowiedzi roślin na stres związany z niedoborem wody.
|
||||
\end{longenum}
|
||||
|
||||
\item Cel modyfikacji
|
||||
\begin{longenum}
|
||||
\item **Nadekspresja genu DREB1A**:
|
||||
\begin{longenum}
|
||||
\item Gen DREB1A koduje czynnik transkrypcyjny, który aktywuje ekspresję genów odpowiedzialnych za ochronę komórek przed odwodnieniem.
|
||||
\item Wzmożona ekspresja DREB1A prowadzi do zwiększenia aktywności szlaków odpowiedzi na stres suszy, co może poprawić zdolność rośliny do przetrwania w warunkach niskiej wilgotności.
|
||||
\end{longenum}
|
||||
\item **Edytowanie sekwencji promotora RD29A**:
|
||||
\begin{longenum}
|
||||
\item Promotor RD29A może być zmodyfikowany, aby zwiększyć ekspresję genu podczas stresu związanego z odwodnieniem.
|
||||
\item Poprzez edycję sekwencji regulatorowej z wykorzystaniem CRISPR, można zwiększyć indukowaną ekspresję genu RD29A, co wzmocni odpowiedź na suszę.
|
||||
\end{longenum}
|
||||
\end{longenum}
|
||||
|
||||
\item Przykładowa modyfikacja z użyciem CRISPR-Cas9
|
||||
\begin{longenum}
|
||||
\item **Ukierunkowanie CRISPR na sekwencję promotora DREB1A**:
|
||||
\begin{longenum}
|
||||
\item Wykorzystując CRISPR-Cas9, można wprowadzić mutację do sekwencji promotora DREB1A, która spowoduje nadekspresję genu.
|
||||
\item Wprowadzenie sekwencji regulatorowej, która zwiększa ekspresję, może uczynić roślinę bardziej odporną na odwodnienie poprzez szybsze i bardziej efektywne uruchamianie mechanizmów obronnych.
|
||||
\end{longenum}
|
||||
\item **Zmiana w sekwencji genowej NHX1**:
|
||||
\begin{longenum}
|
||||
\item Modyfikacja sekwencji kodującej gen NHX1 może zwiększyć wydajność białka transportującego jony, co pomoże lepiej regulować ciśnienie osmotyczne w komórkach.
|
||||
\item Dzięki tej zmianie, Arabidopsis będzie lepiej radzić sobie z niedoborem wody, utrzymując stabilne funkcje komórkowe w warunkach stresu suszy.
|
||||
\end{longenum}
|
||||
\end{longenum}
|
||||
|
||||
\item Przewidywane korzyści z modyfikacji
|
||||
\begin{longenum}
|
||||
\item **Zwiększona tolerancja na suszę**: Modyfikacje genów takich jak DREB1A, RD29A oraz NHX1 poprawią zdolność Arabidopsis do przetrwania w warunkach niskiej dostępności wody, dzięki lepszej regulacji procesów adaptacyjnych.
|
||||
\item **Zachowanie wzrostu i rozwoju w warunkach stresowych**: Dzięki wzmocnionej odpowiedzi na suszę, rośliny będą mogły utrzymać normalny wzrost i rozwój, co jest kluczowe dla rolnictwa w regionach o ograniczonych zasobach wodnych.
|
||||
\end{longenum}
|
||||
\end{longenum}
|
||||
|
||||
|
|
@ -1,65 +0,0 @@
|
|||
\item
|
||||
\begin{center}
|
||||
\fbox{
|
||||
\begin{minipage}{0.9\textwidth}
|
||||
\textbf{Arabidopsis jako roślina modelowa}
|
||||
\end{minipage}
|
||||
}
|
||||
\end{center}
|
||||
\section*
|
||||
|
||||
\begin{longenum}
|
||||
\item Dlaczego Arabidopsis jest rośliną modelową?
|
||||
\begin{longenum}
|
||||
\item \textbf{Genom mały i dobrze poznany}: Arabidopsis thaliana była pierwszą rośliną, której pełny genom został zsekwencjonowany. Dzięki niewielkiemu genomowi (około 135 Mb) oraz dużej liczbie dostępnych narzędzi genetycznych, jest idealnym modelem do badań nad genetyką roślin.
|
||||
\item \textbf{Krótki cykl życiowy}: Arabidopsis ma szybki cykl życiowy (około 6 tygodni od nasion do nasion), co pozwala na szybkie prowadzenie badań i eksperymentów.
|
||||
\item \textbf{Łatwość manipulacji genetycznych}: Dzięki liczbie dostępnych mutantów, linii transgenicznych oraz technik takich jak CRISPR, Arabidopsis jest świetnym systemem modelowym do badania funkcji genów.
|
||||
\end{longenum}
|
||||
|
||||
\item Bazy danych do analizy genów Arabidopsis
|
||||
\begin{longenum}
|
||||
\item \textbf{TAIR (The Arabidopsis Information Resource)}:
|
||||
\begin{longenum}
|
||||
\item Największa baza danych zawierająca informacje o genomie Arabidopsis, sekwencjach genów, mutantach oraz ich funkcjach.
|
||||
\item Można wyszukiwać specyficzne geny oraz przeglądać informacje dotyczące ekspresji genów, fenotypów oraz publikacji.
|
||||
\end{longenum}
|
||||
\item \textbf{ATTED-II}:
|
||||
\begin{longenum}
|
||||
\item Baza danych powiązań genetycznych oparta na współekspresji genów w różnych warunkach.
|
||||
\item Może być używana do identyfikacji genów związanych z odpowiedzią na stresy środowiskowe, takie jak susza.
|
||||
\end{longenum}
|
||||
\item \textbf{ARaport}:
|
||||
\begin{longenum}
|
||||
\item Narzędzie do analizowania i wizualizowania profili ekspresji genów Arabidopsis.
|
||||
\item Umożliwia analizę genów aktywowanych w odpowiedzi na różne czynniki stresowe.
|
||||
\end{longenum}
|
||||
\item \textbf{Gene Ontology (GO)}:
|
||||
\begin{longenum}
|
||||
\item Baza danych klasyfikacji funkcji genów, która pomaga w identyfikacji i analizie genów odpowiedzialnych za określone procesy biologiczne, takie jak odporność na stresy abiotyczne.
|
||||
\end{longenum}
|
||||
\end{longenum}
|
||||
|
||||
\item Geny Arabidopsis związane z odpornością na suszę
|
||||
\begin{longenum}
|
||||
\item \textbf{DREB1A (Dehydration-Responsive Element-Binding Protein 1A)}:
|
||||
\begin{longenum}
|
||||
\item Gen kodujący czynnik transkrypcyjny, który reguluje ekspresję wielu genów odpowiedzi na stres, w tym suszę.
|
||||
\item Wzmacnia odporność na suszę poprzez aktywowanie ścieżek sygnałowych odpowiedzi na niedobór wody.
|
||||
\end{longenum}
|
||||
\item \textbf{RD29A (Response to Dehydration 29A)}:
|
||||
\begin{longenum}
|
||||
\item Jeden z głównych genów markerowych dla stresu suszy, regulowany przez DREB1A.
|
||||
\item Jego ekspresja jest indukowana przez stres związany z odwodnieniem oraz niską temperaturą.
|
||||
\end{longenum}
|
||||
\item \textbf{NHX1 (Sodium/Hydrogen Exchanger 1)}:
|
||||
\begin{longenum}
|
||||
\item Gen kodujący białko transportujące jonów, które pomaga w utrzymaniu homeostazy jonowej w warunkach suszy.
|
||||
\item Jego nadekspresja poprawia zdolność rośliny do przetrwania suszy poprzez zwiększenie magazynowania jonów i regulację ciśnienia osmotycznego.
|
||||
\end{longenum}
|
||||
\item \textbf{P5CS1 (Δ1-Pyrroline-5-Carboxylate Synthetase 1)}:
|
||||
\begin{longenum}
|
||||
\item Kluczowy enzym w syntezie proliny, aminokwasu pełniącego rolę osmolitu, który pomaga roślinom przetrwać stres związany z odwodnieniem.
|
||||
\item Wyższy poziom proliny w komórkach poprawia tolerancję na suszę.
|
||||
\end{longenum}
|
||||
\end{longenum}
|
||||
\end{longenum}
|
||||
|
|
@ -7,35 +7,38 @@
|
|||
}
|
||||
\end{center}
|
||||
|
||||
\vspace{.5cm}
|
||||
\begin{longenum}
|
||||
\item Jak działa CRISPR?
|
||||
\begin{longenum}
|
||||
\item CRISPR działa w połączeniu z enzymem Cas9, który działa jako "nożyczki" tnące DNA. System działa w kilku etapach:
|
||||
\begin{longenum}
|
||||
\item \textbf{Rozpoznanie celu}: CRISPR używa krótkiego odcinka RNA (gRNA - guide RNA), który jest komplementarny do docelowej sekwencji DNA. RNA prowadzi enzym Cas9 do odpowiedniego miejsca w genomie.
|
||||
\item \textbf{Cięcie DNA}: Po dotarciu do docelowej sekwencji, Cas9 przecina dwie nici DNA, tworząc przerwę.
|
||||
\item \textbf{Naprawa DNA}: Komórki próbują naprawić uszkodzoną sekwencję DNA, co może skutkować wprowadzeniem mutacji lub dodaniem nowego genu.
|
||||
\end{longenum}
|
||||
\end{longenum}
|
||||
\vspace{1cm}
|
||||
\textbf{Główne komponenty CRISPR:}
|
||||
\begin{itemize}
|
||||
\item \textbf{Białko Cas9:}
|
||||
\begin{itemize}
|
||||
\item Enzym, który przecina DNA.
|
||||
\item Rozpoznaje specyficzne miejsca w genomie.
|
||||
\item Działa jak molekularne nożyczki \cite{jinekProgrammableDualRNAGuidedDNA2012}.
|
||||
\end{itemize}
|
||||
\item \textbf{Przewodnik RNA (gRNA):}
|
||||
\begin{itemize}
|
||||
\item Krótki fragment RNA.
|
||||
\item Prowadzi białko Cas9 do docelowego DNA.
|
||||
\item Wiąże się z wybraną sekwencją DNA \cite{jinekProgrammableDualRNAGuidedDNA2012}.
|
||||
\end{itemize}
|
||||
\end{itemize}
|
||||
|
||||
\vspace{0.5cm}
|
||||
\item Zastosowania CRISPR
|
||||
\begin{longenum}
|
||||
\item CRISPR ma szerokie zastosowania w różnych dziedzinach:
|
||||
\begin{longenum}
|
||||
\item \textbf{Biotechnologia}: Edycja genów roślin, aby poprawić ich odporność na choroby, suszę, czy zwiększyć plony.
|
||||
\item \textbf{Medycyna}: Potencjalne terapie genowe do leczenia chorób dziedzicznych, takich jak mukowiscydoza czy anemia sierpowata.
|
||||
\item \textbf{Badania naukowe}: Badania nad funkcją genów i modelowanie chorób genetycznych.
|
||||
\end{longenum}
|
||||
\end{longenum}
|
||||
|
||||
\vspace{0.5cm}
|
||||
\item Przykład w kontekście Arabidopsis
|
||||
\begin{longenum}
|
||||
\item CRISPR może być użyty do wprowadzenia modyfikacji w genach Arabidopsis odpowiedzialnych za reakcje na stres suszy.
|
||||
\item Można zaprojektować gRNA, aby wycelować w specyficzne geny regulujące tolerancję na suszę, a następnie użyć CRISPR do knock-out lub zmodyfikowania tych genów w celu poprawienia adaptacji roślin do warunków niedoboru wody.
|
||||
\item Dzięki CRISPR można tworzyć bardziej odporne odmiany roślin, co ma znaczący wpływ na rolnictwo, zwłaszcza w obliczu zmian klimatycznych.
|
||||
\end{longenum}
|
||||
\end{longenum}
|
||||
\vspace{1cm}
|
||||
\textbf{Zastosowania CRISPR:}
|
||||
\begin{itemize}
|
||||
\item Modyfikacja genów:
|
||||
\begin{itemize}
|
||||
\item Knock-out genów (wyłączenie funkcji genu).
|
||||
\item Wprowadzenie mutacji.
|
||||
\item Zmiana sekwencji DNA.
|
||||
\end{itemize}
|
||||
\end{itemize}
|
||||
|
||||
\vspace{1cm}
|
||||
\textbf{Przykłady zastosowań:}
|
||||
\begin{itemize}
|
||||
\item Zwiększenie odporności roślin na suszę \cite{montecilloCRISPRCas9SystemPlant2020}.
|
||||
\item Poprawa cech rolniczych zwierząt.
|
||||
\item Terapia genowa u ludzi \cite{doudnaNewFrontierGenome2014}.
|
||||
\end{itemize}
|
||||
|
|
|
|||
|
|
@ -51,32 +51,3 @@
|
|||
langid = {english},
|
||||
file = {/home/user/Zotero/storage/JJPA5C9W/Montecillo et al. - 2020 - CRISPR-Cas9 System for Plant Genome Editing Current Approaches and Emerging Developments.pdf}
|
||||
}
|
||||
|
||||
@article{source56,
|
||||
author = {TAIR Database},
|
||||
title = {Gene information for DREB1A (AT4G25480)},
|
||||
year = {2024},
|
||||
url = {https://uat.arabidopsis.org/servlets/TairObject?type=locus&name=At4g25480}
|
||||
}
|
||||
|
||||
@article{source57,
|
||||
author = {Phytozome},
|
||||
title = {Gene report for AT4G25480 - Arabidopsis thaliana},
|
||||
year = {2024},
|
||||
url = {https://phytozome-next.jgi.doe.gov/report/gene/Athaliana_TAIR10/AT4G25480}
|
||||
}
|
||||
|
||||
@article{source58,
|
||||
author = {UniProt},
|
||||
title = {DREB1A Protein Information},
|
||||
year = {2024},
|
||||
url = {https://www.uniprot.org/uniprotkb/Q9M0L0/entry}
|
||||
}
|
||||
|
||||
@article{source59,
|
||||
author = {Ensembl Genomes},
|
||||
title = {Gene summary: DREB1A (AT4G25480)},
|
||||
year = {2024},
|
||||
url = {https://plants.ensembl.org/Arabidopsis_thaliana/Gene/Summary?g=AT4G25480}
|
||||
}
|
||||
|
||||
|
|
|
|||
Loading…
Reference in New Issue