From 5b8592fbd75de93390ca79ec9de3b28b0e4b9c53 Mon Sep 17 00:00:00 2001
From: mpabi <mpabi@qstack.pl>
Date: Sun, 9 Jun 2024 17:39:24 +0200
Subject: [PATCH] u

---
 gfp-old.py | 9 ++++++++-
 1 file changed, 8 insertions(+), 1 deletion(-)

diff --git a/gfp-old.py b/gfp-old.py
index e759c73..999a65e 100644
--- a/gfp-old.py
+++ b/gfp-old.py
@@ -31,6 +31,8 @@ def load_local_sequence(file_path):
         record = SeqIO.read(file, "genbank")
     return record
 
+#%%
+
 # Ścieżki do lokalnych plików
 gfp_file_path = 'gfp_sequence.gb'
 plasmid_file_path = 'plasmid_sequence.gb'
@@ -65,9 +67,11 @@ else:
 # Dodanie sekwencji kodującej His6
 his6_tag = "CACCACCACCACCACCAC"  # Sekwencja kodująca 6x histydynę (His6)
 
+#%%
 # Sekwencja białka GFP z His6 tagiem na końcu
-gfp_seq_with_his6 = gfp_seq + his6_tag
+t = gfp_seq_with_his6 = gfp_seq + his6_tag
 
+#%%
 # Projektowanie starterów
 forward_primer = gfp_seq[:20]  # Pierwsze 20 nukleotydów sekwencji GFP
 reverse_primer = str(Seq(gfp_seq_with_his6[-20:]).reverse_complement())  # Ostatnie 20 nukleotydów + His6
@@ -75,10 +79,13 @@ reverse_primer = str(Seq(gfp_seq_with_his6[-20:]).reverse_complement())  # Ostat
 print(f"Forward Primer: {forward_primer}")
 print(f"Reverse Primer: {reverse_primer}")
 
+
+#%%
 # Miejsca cięcia restryktazy
 enzymes = RestrictionBatch([EcoRI, BamHI, HindIII])
 restriction_sites = enzymes.search(record.seq)
 
+#%%
 # Utwórz diagram plazmidu
 diagram = GenomeDiagram.Diagram("pET-28a(+) Plasmid Map")
 track = diagram.new_track(1, name="Annotated Features", greytrack=True)