67 lines
3.5 KiB
Plaintext
67 lines
3.5 KiB
Plaintext
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LOCUS SCU87974 818 bp mRNA linear SYN 24-FEB-1997
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DEFINITION Synthetic construct modified green fluorescent protein GFP5-ER
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(mgfp5-ER) mRNA, complete cds.
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ACCESSION U87974
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VERSION U87974.1
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KEYWORDS .
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SOURCE synthetic construct
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ORGANISM synthetic construct
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other sequences; artificial sequences.
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REFERENCE 1 (bases 1 to 818)
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AUTHORS Siemering,K.R., Golbik,R., Sever,R. and Haseloff,J.
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TITLE Mutations that suppress the thermosensitivity of green fluorescent
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protein
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JOURNAL Curr. Biol. 6 (12), 1653-1663 (1996)
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PUBMED 8994830
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REFERENCE 2 (bases 1 to 818)
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AUTHORS Haseloff,J., Siemering,K.R., Prasher,D. and Hodge,S.
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TITLE Removal of a cryptic intron and subcellular localisation of green
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fluorescent protein are required to mark transgenic Arabidopsis
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plants brightly
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JOURNAL Proc. Natl. Acad. Sci. U.S.A. (1997) In press
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REFERENCE 3 (bases 1 to 818)
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AUTHORS Siemering,K.R., Golbik,R., Sever,R. and Haseloff,J.
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TITLE Direct Submission
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JOURNAL Submitted (31-JAN-1997) Division of Cell Biology, MRC Laboratory of
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Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
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FEATURES Location/Qualifiers
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source 1..818
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/organism="synthetic construct"
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/mol_type="mRNA"
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/db_xref="taxon:32630"
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gene 1..818
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/gene="mgfp5-ER"
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CDS 21..812
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/gene="mgfp5-ER"
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/note="contains codon usage changes that disrupt a cryptic
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plant intron, mutations that increase the thermotolerance
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and change the spectral characteristics of the protein, and
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sequences that code for signal peptides that result in
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retention of the protein in the plant endoplasmic
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reticulum"
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/codon_start=1
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/transl_table=11
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/product="modified green fluorescent protein GFP5-ER"
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/protein_id="AAB47999.1"
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/translation="MKTNLFLFLIFSLLLSLSSAEFSKGEELFTGVVPILVELDGDVNG
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HKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDF
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FKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLE
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YNYNSHNVYIMADKQKNGIKANFKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYL
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STQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKHDEL"
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ORIGIN
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1 ggatccaagg agatataaca atgaagacta atctttttct ctttctcatc ttttcacttc
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61 tcctatcatt atcctcggcc gaattcagta aaggagaaga acttttcact ggagttgtcc
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121 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
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181 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
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241 tacctgttcc atggccaaca cttgtcacta ctttctctta tggtgttcaa tgcttttcaa
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301 gatacccaga tcatatgaag cggcacgact tcttcaagag cgccatgcct gagggatacg
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361 tgcaggagag gaccatcttc ttcaaggacg acgggaacta caagacacgt gctgaagtca
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421 agtttgaggg agacaccctc gtcaacagga tcgagcttaa gggaatcgat ttcaaggagg
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481 acggaaacat cctcggccac aagttggaat acaactacaa ctcccacaac gtatacatca
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541 tggccgacaa gcaaaagaac ggcatcaaag ccaacttcaa gacccgccac aacatcgaag
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601 acggcggcgt gcaactcgct gatcattatc aacaaaatac tccaattggc gatggccctg
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661 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
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721 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
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781 tggatgaact atacaaacat gatgagcttt aagagctc
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//
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